rock1 polyclonal antibody Search Results


anti rock1 antibody bs 1166r  (Bioss)
91
Bioss anti rock1 antibody bs 1166r
RAB14 knockdown disrupts cytoplasmic actin assembly in mouse oocytes. (A) Representative images of actin filament distribution at the oocyte cortex and cytoplasm in the control group, RAB14‐KD group and rescue group. Red, F‐actin. Bar = 20 μm. (B) No significant difference was observed between control oocytes, Rab14 siRNA‐injected oocytes and rescue oocytes for the F‐actin fluorescent intensities at the cortex ( P > .1); however, F‐actin fluorescent intensities in the cytoplasm were decreased in the Rab14 siRNA‐injected oocytes compared with the control and rescue oocytes. *, significant difference ( P < .05). (C) Quantitative analysis of the relative intensity of ARP2, N‐WASP, <t>ROCK1</t> and p‐cofilin in oocytes by Western blot. The results indicated that ROCK1 and p‐cofilin protein expression were significantly reduced in oocytes after RAB14 depletion. **, significant difference ( P < .01); *, significant difference ( P < .05). (D) Representative images of ROCK1 in the control and RAB14‐KD oocytes. ROCK1 fluorescent intensities decreased in the RAB14‐KD oocytes compared with the control oocytes. *, significant difference ( P < .05). Bar = 20 μm
Anti Rock1 Antibody Bs 1166r, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rock1+polyclonal+antibody/ROCK1+Polyclonal+Antibody/pmc08450121-42-3-12
Average 91 stars, based on 1 article reviews
anti rock1 antibody bs 1166r - by Bioz Stars, 2026-09
91/100 stars
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rock1 antibody  (OriGene)
90
OriGene rock1 antibody
Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, <t>ROCK1,</t> p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05
Rock1 Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rock1+polyclonal+antibody/ROCK1+Rabbit+Polyclonal+Antibody/pm28415713-187-5-7
Average 90 stars, based on 1 article reviews
rock1 antibody - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit polyclonal cy5 conjugated rock antibody  (Bioss)
92
Bioss rabbit polyclonal cy5 conjugated rock antibody
Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, <t>ROCK1,</t> p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05
Rabbit Polyclonal Cy5 Conjugated Rock Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rock1+polyclonal+antibody/ROCK1+Polyclonal+Antibody%2C+Cy5+Conjugated/pmc08962687-128-0-9
Average 92 stars, based on 1 article reviews
rabbit polyclonal cy5 conjugated rock antibody - by Bioz Stars, 2026-09
92/100 stars
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anti rock-2 rabbit rabbit bs-4630r ab66320 bioss  (Bioss)
92
Bioss anti rock-2 rabbit rabbit bs-4630r ab66320 bioss
Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, <t>ROCK1,</t> p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05
Anti Rock 2 Rabbit Rabbit Bs 4630r Ab66320 Bioss, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rock1+polyclonal+antibody/ROCK1(Thr455+Ser456)+Polyclonal+Antibody/pm28821742-100-85-90
Average 92 stars, based on 1 article reviews
anti rock-2 rabbit rabbit bs-4630r ab66320 bioss - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
rock 2  (EnoGene Inc)
90
EnoGene Inc rock 2
Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, <t>ROCK1,</t> p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05
Rock 2, supplied by EnoGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rock1+polyclonal+antibody/rabbit+polyclonal+primary+antibodies+against+rock1+rock2/pmc04377486-45-70-76
Average 90 stars, based on 1 article reviews
rock 2 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rock1 rabbit polyclonal antibody  (OriGene)
90
OriGene rock1 rabbit polyclonal antibody
Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, <t>ROCK1,</t> p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05
Rock1 Rabbit Polyclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rock1+polyclonal+antibody/ROCK1+Rabbit+Polyclonal+Antibody/origene___ta325814
Average 90 stars, based on 1 article reviews
rock1 rabbit polyclonal antibody - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit anti- rock1 polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human ROCK1 Polyclonal Antibody
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anti-rock1 (n-terminal) polyclonal antibody  (Creative BioMart)
N/A
DPABH-05630 is predicted not to cross-react with other ROCK protein family members.Phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell
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rock1 polyclonal antibody, abby fluor 488 conjugated  (Bioss)
N/A
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility
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rock1 polyclonal antibody, abby fluor 555 conjugated  (Bioss)
N/A
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility
   Buy from Supplier
rock1(thr455 ser456) polyclonal antibody, abby fluor 405 conjugated  (Bioss)
N/A
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility
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Image Search Results


RAB14 knockdown disrupts cytoplasmic actin assembly in mouse oocytes. (A) Representative images of actin filament distribution at the oocyte cortex and cytoplasm in the control group, RAB14‐KD group and rescue group. Red, F‐actin. Bar = 20 μm. (B) No significant difference was observed between control oocytes, Rab14 siRNA‐injected oocytes and rescue oocytes for the F‐actin fluorescent intensities at the cortex ( P > .1); however, F‐actin fluorescent intensities in the cytoplasm were decreased in the Rab14 siRNA‐injected oocytes compared with the control and rescue oocytes. *, significant difference ( P < .05). (C) Quantitative analysis of the relative intensity of ARP2, N‐WASP, ROCK1 and p‐cofilin in oocytes by Western blot. The results indicated that ROCK1 and p‐cofilin protein expression were significantly reduced in oocytes after RAB14 depletion. **, significant difference ( P < .01); *, significant difference ( P < .05). (D) Representative images of ROCK1 in the control and RAB14‐KD oocytes. ROCK1 fluorescent intensities decreased in the RAB14‐KD oocytes compared with the control oocytes. *, significant difference ( P < .05). Bar = 20 μm

Journal: Cell Proliferation

Article Title: RAB14 GTPase is essential for actin‐based asymmetric division during mouse oocyte maturation

doi: 10.1111/cpr.13104

Figure Lengend Snippet: RAB14 knockdown disrupts cytoplasmic actin assembly in mouse oocytes. (A) Representative images of actin filament distribution at the oocyte cortex and cytoplasm in the control group, RAB14‐KD group and rescue group. Red, F‐actin. Bar = 20 μm. (B) No significant difference was observed between control oocytes, Rab14 siRNA‐injected oocytes and rescue oocytes for the F‐actin fluorescent intensities at the cortex ( P > .1); however, F‐actin fluorescent intensities in the cytoplasm were decreased in the Rab14 siRNA‐injected oocytes compared with the control and rescue oocytes. *, significant difference ( P < .05). (C) Quantitative analysis of the relative intensity of ARP2, N‐WASP, ROCK1 and p‐cofilin in oocytes by Western blot. The results indicated that ROCK1 and p‐cofilin protein expression were significantly reduced in oocytes after RAB14 depletion. **, significant difference ( P < .01); *, significant difference ( P < .05). (D) Representative images of ROCK1 in the control and RAB14‐KD oocytes. ROCK1 fluorescent intensities decreased in the RAB14‐KD oocytes compared with the control oocytes. *, significant difference ( P < .05). Bar = 20 μm

Article Snippet: A rabbit polyclonal anti‐ROCK1 antibody (bs‐1166R) (for immnuofluorescence staining) was purchased from Bioss.

Techniques: Injection, Western Blot, Expressing

Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, ROCK1, p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05

Journal: Oncotarget

Article Title: miR-135a inhibits tumor metastasis and angiogenesis by targeting FAK pathway.

doi: 10.18632/oncotarget.16098

Figure Lengend Snippet: Figure 2: FAK is a target gene of miR-135a. (A) The binding site of miR-135a in the FAK 3′UTR and the skeleton of FAK 3′UTR luciferase reporter vector. (B) The protein expression of FAK, p-ERK, ERK, ROCK1, p-LIMK1 and LIMK1 in SGC-7901 and BGC-823 stable cell lines are examined by Western Blot. (C) Relative expression of secreted VEGFA in the culture supernatant of cancer cells is detected by ELISA assay. (D) The protein expression of phosphorylated FAK (pTyr397) in cells that treated with conditioned medium from control or miR-135a overexpressing stable cell lines. (E) miR-135a overexpressing cells were transfected with 200 nM miRNA inhibitor or control RNA, then FAK protein level was examined with Western blot assay. (F) The effect of miR-135a mimics on the luciferase activity of FAK 3′UTR wild type (WT) and deletion type (DEL) are detected by luciferase reporter assay. * p < 0.05

Article Snippet: FAK1 (Cell signaling technology, USA), ROCK1 antibody (Origene, USA), p-LIMK1 (Cell signaling technology, USA), GAPDH antibody (Kangchen, China), p53 (Santa Cruz, USA) and Flag (or DKK) antibody (Abmart, China) were used.

Techniques: Binding Assay, Luciferase, Plasmid Preparation, Expressing, Stable Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, Control, Transfection, Activity Assay, Reporter Assay